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Capillary electrophoresis employed for quantitative characterization of peptide interactions with small ions and cyclodextrins
Kašička, Václav ; Ehala, Sille ; Šolínová, Veronika ; Schimperková, Tereza ; Sázelová, Petra ; Koval, Dušan ; Makrlík, E.
Capillary affinity electrophoresis has been applied to investigate quantitatively two types of non-covalent peptide interactions: i) complexes of pseudopeptide valinomycin with ammonium ion and alkali metal ions, Li+, Na+, K+, Rb+ and Cs+, in methanol, and ii) complexes of enantiomers of dipeptide beta alanyl-D,L-tyrosine and its derivatives with chiral selector, 2-hydroxypropyl-beta-cyclodextrin, in water.
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Investigation of peptide complexes with small cations and cyclodextrins by capillary affinity electrophoresis
Kašička, Václav ; Ehala, Sille ; Šolínová, Veronika ; Schimperková, Tereza ; Sázelová, Petra ; Koval, Dušan
Capillary affinity electrophoresis was applied to quantitative evaluation of the strength of the complexes of i) valinomycin, macrocyclic dodecadepsipeptide ionophore, with small cations (ammonium and alkali metal ions, Li+, Na+, K+, Rb+ and Cs+) in methanol, and ii) enantiomers of dipeptide beta-alanyl-D,L-tyrosine and its derivatives with a chiral selector, 2 hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD), in water.
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Characterization of valinomycin binding with alkali metal ions by capillary affinity electrophoresis and density functional theory
Ehala, Sille ; Dybal, Jiří ; Makrlík, E. ; Kašička, Václav
Capillary affinity electrophoresis (CAE) and quantum mechanical density functional theory (DFT) have been applied to the study of non-covalent binding of valinomycin (Val), an antibiotic dodecadepsipeptide ionophore, with alkali metal ions, Li+, Na+, K+, Rb+ and Cs+, in methanol. The binding constants (Kb) of Val-alkali metal ion complexes were evaluated by CAE. The most probable structures of the Val-alkali metal ion complexes were proposed by DFT.
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